- Research article
- Open Access
Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
© Kanaji et al.; licensee BioMed Central Ltd. 2014
- Received: 20 November 2013
- Accepted: 24 September 2014
- Published: 8 October 2014
Metal-on-metal prostheses undergo wear and corrosion, releasing soluble ions and wear particles into the surrounding environment. Reports described early failures of the metal-on-metal prostheses, with histologic features similar to a Type IV immune response. Mechanisms by which metal wear products and metal ion causing this reaction are not completely understood, and the effects of metal ions on osteocytes, which represent more than 95% of all the bone cells, have not been also studied. We hypothesized that soluble metal ions released from the cobalt-chromium-molybdenum (Co-Cr-Mo) prosthesis may have cytotoxic effect on osteocytes.
MLO-Y4 osteocytes were treated with various metal ion solutions for 24 and 48 h. The effect of ion treatment on cytotoxicity was assessed by WST-1 reagents and cell death ELISA. Morphological changes were analyzed by a phase-contrast microscope or fluorescent microscope using Hoechst 33342 and propidium iodine staining.
Cr and Mo ions did not cause cell death under 0.50 mM, highest concentration studied, whereas Co and Ni ions had significant cytotoxic effect on MLO-Y4 cells at concentrations grater than 0.10 mM and at 0.50 mM, respectively, in a dose-dependent manner. According to the ELISA data, osteocytes treated with Co ions were more susceptible to necrotic than apoptotic cell death, while Ni ions caused osteocyte apoptosis. The morphological assays show that cells treated with Co and Ni ions at high concentration were fewer in number and rounded. In addition, fluorescent images showed a marked reduction in live cells and an increase in dead osteocytes treated with Co and Ni ions at high concentration.
Metal ions released from metal-on-metal bearing surfaces have potentially cytotoxic effects on MLO-Y4 osteocytes, in vitro.
- Metal-on-metal bearing surfaces
- Metal ions
In 2005, approximately 250,000 total hip arthroplasties were performed in the United States , and according to a recent report, more than 30% had metal-on-metal bearings . The use of a metal-on-metal bearing in total hip arthroplasty and hip resurfacing arthroplasty is advantageous in terms of lower volumetric wear rate (by 20 to 100 times) in comparison to conventional metal-on-polyethylene bearings . Other benefits include high fracture toughness and the ability to use large femoral heads, which reduces the risk of postoperative instability . However, it has been well documented that metal-on-metal prostheses in contact with host tissue undergo wear and corrosion, releasing soluble ions and wear particles into the surrounding environment . Elevated levels of metal ions, such as cobalt and chromium, have been detected in the body fluids; serum, whole blood, synovial fluid, and tissues obtained from subjects with well-functioning metal-on-metal implants -. It is unclear to what extent exposure to soluble metal ions released from implants over the long run can lead to; it may lead to a poor outcome such as aseptic loosening. The high usage (44.8%) of metal-on-metal bearing surfaces in patients below 55 years old  has raised concerns about the long-term biological effects of metals , including toxicity and sensitivity reactions.
Aseptic loosening is mostly attributed to wear debris particles. Cells of the monocyte/macrophage lineage play a primary role by phagocytosing wear particles that lead to the activation and release of pro-inflammatory mediators which accelerate bone resorption around the implant . While other cell types such as lymphocytes, endothelial cells, fibroblasts, bone marrow-derived mesenchymal cells, osteoblasts, and osteoclasts contribute to the pathology, according to numerous studies , osteocytes, which represent more than 95% of all the bone cells, have not been examined. Moreover, the precise etiology of aseptic loosening still remains unclear.
Osteocytes are terminally differentiated cells of the osteoblast lineage that have become embedded in a mineralized bone matrix and may send signals that regulate bone modeling and remodeling . Although the role of osteocytes remained unknown for a long time, numerous reports highlight their role by demonstrating the linkage between osteocyte death/apoptosis to the control of local bone resorption -. More recently, it has been suggested that osteocytes may be involved in the inflammatory response and subsequent periprosthetic bone resorption following total joint arthroplasty -. Metal (cobalt-chromium-molybdenum; Co-Cr-Mo) wear particles were able to stimulate production of the pro-inflammatory cytokine by osteocytes that could lead to osteocyte apoptosis, in vitro. However, the effects of soluble metal ions on osteocytes have not been studied. We hypothesized that soluble metal ions released from Co-Cr-Mo prostheses will have a cytotoxic effect on osteocytes at clinically relevant concentrations -,-, suggesting a role of osteocytes in the pathophysiology of implant failure.
MLO-Y4, a murine long bone-derived osteocytic cell line, derived from 14-day-old transgenic mice containing the SV40 large T-antigen driven by the osteocalcin promoter 23] was kindly provided by Dr. Lynda F. Bonewald (University of Missouri-Kansas City, Kansas City, MO, USA) for use in this study. The MLO-Y4 cell line displays an osteocyte phenotype. Cells were cultured at 37 C, 5% CO2, 95% air in α-MEM supplemented with 2.5% fetal bovine serum, 2.5% calf serum, and antibiotics on tissue culture plastic dishes coated with rat tail collagen (BD Biosciences, San Jose, CA, USA) as previously described . Cells were plated at a density of 1.0 × 105 cells/well in 12-well culture plates.
Soluble metal solutions
After a 24-hr pre-culture period, the MLO-Y4 cells were treated with four concentrations of sodium (Na+), cobalt (Co2+), chromium (Cr3+), molybdenum (Mo5+), and nickel (Ni2+) chloride solutions at 0.00 (control), 0.05, 0.10, and 0.50 mM, respectively. These concentrations were chosen based upon a previous study utilizing osteoblasts in which Co and Ni at 0.50 mM (clinically relevant concentrations) demonstrated cell toxicity . Except for Na which was used for comparison, all the metals tested were components of Co-Cr-Mo implant alloys. Stock metal ion solutions (10 mM) were prepared by dissolving sodium (NaCl), cobalt (CoCl2), chromium (CrCl3), molybdenum (MoCl5), and nickel (NiCl2) chloride powders (Sigma-Aldrich, St. Louis, MO, USA) with double-deionized water (Milli-Q water system; Millipore, Bedford, MA, USA), filter-sterilized before use as described previously  and added to cell cultures to the final concentrations listed above.
Cell proliferation reagent WST-1 (Roche Diagnostics, Indianapolis, IN, USA) was used to investigate the cytotoxic effect of the metal ions after 48 hr. In brief, after a 48-hr metal ion treatment, a ready-to-use WST-1 solution was added and incubation continued for 2 hr at 37°C. The reaction product was measured at 450 nm with the reference wavelength at 630 nm by a microplate reader (BioTek, Winooski, VT, USA).
Detection of apoptosis and necrosis
The Cell Death Detection ELISAPLUS photometric enzyme immunoassay kit (Roche Diagnostics, Indianapolis, IN, USA) was used to analyze the apoptotic and necrotic cell death quantitatively after 24 hr. The kit utilizes two monoclonal antibodies directed against DNA and histones, respectively, to allow the specific determination of mono- and oligo-nucleosomes in the cytoplasmatic fraction (apoptosis) or in the supernatant (necrosis) of cells.
Morphological analyses were done under a phase-contrast microscope (Nikon, Tokyo, Japan) and fluorescent microscope (Nikon, Tokyo, Japan) using propidium iodide (Sigma-Aldrich, St. Louis, MO, USA) and Hoechst 33342 (Biotium, Hayward, CA, USA). The propidium iodide was used to identify dead cells while Hoechst 33342 was used to stain the total (dead and healthy) cells. The charged dye propidium iodide is excluded by cells with intact membranes, whereas Hoechst 33342 stains DNA in all cells. In brief, MLO-Y4 cells were washed once in PBS and incubated in a staining solution which contains propidium iodide (10 μM) and Hoechst 33342 (25 μM) at room temperature for 15 min. Cells were washed with PBS and then analyzed by fluorescence microscopy using filters for rhodamine (red) and 4',6-diamidino-2-phenylindole dihydrochloride (DAPI; blue).
For quantitative results, all values are presented as mean ± standard deviation of at least four replicates. These values were analyzed with two-way ANOVA and the Bonferroni/Dunn test as a post hoc test for all groups using GraphPad Instat® software (GraphPad Software Inc., San Diego, CA, USA). Significance was defined as probability values less than 0.05 (p <0.05).
Source of funding
This study was supported by a grant from NIH (R21AR054171 and T32AR052272), the Rush University Committee on Research grant, and the Grainger Foundation.
Detection of apoptosis and necrosis
The objective of this study was to determine the osteocyte response to soluble metal ions that are reported to be released from metal-on-metal (Co-Cr-Mo) implants -. For this, we used the MLO-Y4 cells, an osteocyte cell line, which shows numerous osteocytic characteristics . Utilizing this cell line has the advantage of providing a uniform population of well-characterized cells, unlike bone-derived primary cells, which contain a number of different cell types.
The present study demonstrated that particular metal ions that are reported to be released from a metal-on-metal bearing surface - have effects on osteocytes that are metal-specific. Co and Ni ions, demonstrated a cytotoxic effect on MLO-Y4 osteocytes while Cr and Mo ions had a proliferative effect. This is the first report to show that metal ion solutions can have cytotoxic effects on osteocytes in vitro. Among all metal ion solutions studied, Co and Ni ions had a significant cytotoxic effect on MLO-Y4 cells at concentrations above 0.05 mM and at 0.10 mM, respectively, in a dose-dependent manner. The results from the cell death ELISA demonstrate that Co ion exposure induced osteocyte necrosis rather than apoptosis. On the other hand, Ni ion exposure induced osteocytes to apoptosis. Morphological assays support the results from the cytotoxic assays by showing fewer cells overall and cells with rounded morphology when treated with Co and Ni ions at high concentration (0.50 mM). Additionally, fluorescent images provide qualitative evidence of a decrease in total cell number and higher numbers of dead osteocytes treated with Co and Ni ions at high concentration (0.50 mM).
Our findings are in agreement with previous studies that showed the negative effects of metal ions using different cell types, such as fibroblasts, osteoblasts, lymphocytes, and macrophages ,,-. Caicedo et al. reported that Ni ions induced T cell apoptosis at 0.10 mM and Co ion was the most toxic metal, reducing the T cell viability at 0.50 mM. Whereas the negative effects of Cr and Mo were mostly induced at the higher concentration (5.00 mM) . Similarly, Hallab et al. demonstrated cytotoxic effects of Co and Ni ions on osteoblasts, fibroblasts, and lymphocytes at a low dose (0.01 to 2.00 mM), whereas the same level of cytotoxicity from Cr and Mo ions on each cell types were achieved at approximately 10-fold higher dose (1.00 to >10.00 mM) . Several other studies also reported Co ion as more toxic for the induction of osteoblast and macrophage cell death compared to Cr ion -.
It has been reported that osteocytes exist near the implant surface, in vivo,. A study using conventional and high-voltage transmission electron microscopy demonstrated that osteocytes were routinely observed at the bone-implant surface and extend cellular processes directly onto the implant surface through their canaliculi . However, the clinical relevance of metal-ion-induced osteocyte toxicity in vitro remains uncertain. Information of the concentration of metal ions in periprosthetic bone is limited when compared to other human body fluids and tissues. Busse et al. successfully detected a considerable amount of heavy metal in the periprosthetic mineralized bone tissue . By utilizing proton-induced X-ray emission microanalysis, Co concentration in the periprosthetic mineralized bone tissue (Gruen zone 7) was 38 to 413 parts per million (ppm) , although no distinction was made between entrapped particles and homogeneously distributed ions in the bone matrix. Converting ppm concentrations to mM : ppm/molecular weight (Co: 58.93) = mM, the concentration of Co in the bone matrix will be greater than 0.64 mM. This information indicates, at least for Co, that the cytotoxic effect in osteocytes in the current study which was observed beyond 0.10 mM may be clinically relevant.
Our current findings may have important implications regarding the potential role of osteocytes in periprosthetic bone resorption, since several studies have shown that osteocyte apoptosis contributes to the control of local bone resorption by sending signals to recruit osteoclasts. Osteocytes, historically, have been considered to be metabolically inactive cells  with little attention given to them, compared with studies on osteoblasts and osteoclasts . However, there is now evidence that osteocytes regulate bone formation and resorption -. Several studies have demonstrated that bone fatigue-induced microdamages or unloading leads osteocytes to undergo apoptosis and the involved bone segment is subsequently resorbed by osteoclasts -. Cardoso et al. demonstrated that rats treated with the pan-caspase inhibitor, an apoptosis inhibitor, completely blocked both fatigue-induced osteocyte apoptosis and the activation of bone resorption by osteoclasts, suggesting that apoptotic events following a fatigue-induced microdamage may play a substantial role in determining the course of bone remodeling . In addition, Tatsumi et al. generated osteocyte-ablated transgenic mice and demonstrated significantly lower bone strength with increased intracortical porosity and microfractures, osteoblastic dysfunction, and trabecular bone loss with microstructural deterioration and adipose tissue proliferation in the bone marrow space . These results support previous findings in which osteocyte apoptosis contributes to the control of local bone resorption by sending signals to recruit osteoclasts. Our data support the concept that factors other than excessive loading or unloading may induce osteocytes to undergo apoptosis and activate bone resorption.
The current study has several limitations that will require further research. Although, it has been reported that osteocytes exist near the implant surface , and that considerable amount (0.64 to 7.00 mM) of heavy metal (Co) was detected in the periprosthetic mineralized bone tissue , the exact concentration of the metal ions which osteocytes experience in vivo remains unknown. The concentrations of the metal ions in this study were decided by an assumption that the matrix-embedded osteocytes experience the amount of metals that were detected in the bone matrix. Further experiments should be conducted to elucidate the concentration of metal ions which osteocytes are exposed in vivo. Moreover, currently, direct evidence showing the ability of metal ions released from metal implants to induce osteocyte apoptosis and trigger bone remodeling in vivo is not available. However, it has been reported that osteocyte apoptosis induced by weightlessness or a bone fatigue microdamage triggers bone remodeling in vivo,. Therefore, evidence whether osteocyte apoptosis induced by metal ions stimulate bone remodeling and their capability to initiate subsequent implant loosening awaits future study. Another related limitation in this type of experiment involves use of cell lines as an approximation of osteocytes in vivo. For this, at this stage, our preliminary findings are only able to propose osteocytes as a possible cell type that may contribute to implant failure. Lastly, the mechanism is unknown for how osteocytes recognize/sense the metal ions. To date, whether cytotoxic effects of Co and Ni, observed in our study, require intracellular uptake of these metal ions or are mediated via surface-based receptors or a destabilization of the cell membrane integrity is unknown. This remains to be investigated.
In conclusion, we have demonstrated that metal ions that are reported to be released from metal-on-metal bearing surfaces - have potentially cytotoxic effects on osteocytes. These effects together with recent knowledge on the connection between osteocyte apoptosis and initiation of bone resorption suggest that osteocytes may play an important role in regulating periprosthetic bone resorption following total hip arthroplasty by becoming apoptotic upon metal challenge. These findings warrant further in vivo study of the degree of osteocyte involvement in the pathogenesis of aseptic loosening.
AK, VO, and KS conducted the experiments and analyzed the data. AK and VO drafted the manuscript. MSC and NJH participated in the design of the study. ASV and KS designed the experiments and modified the manuscript. DRS, NJH, and TY provided valuable comments for manuscript drafting. All authors read and approved the final manuscript.
The authors gratefully thank Dr. Lynda F. Bonewald, University of Missouri-Kansas City, for providing the MLO-Y4 cells.
- De Frances CJ, Cullen KA, Kozak LJ: National Hospital Discharge Survey: 2005 annual summary with detailed diagnosis and procedure data. Vital Health Stat 13. 2007, 13: 1-209.Google Scholar
- Bozic KJ, Kurtz S, Lau E, Ong K, Chiu V, Vail TP, Rubash HE, Berry DJ: The epidemiology of bearing surface usage in total hip arthroplasty in the United States. J Bone Joint Surg Am. 2009, 91: 1614-1620. 10.2106/JBJS.H.01220.View ArticlePubMedGoogle Scholar
- Silva M, Heisel C, Schmalzried TP: Metal-on-metal total hip replacement. Clin Orthop Relat Res. 2005, 430: 53-61. 10.1097/01.blo.0000149995.84350.d7.View ArticlePubMedGoogle Scholar
- Jacobs JJ, Urban RM, Hallab NJ, Skipor AK, Fischer A, Wimmer MA: Metal-on-metal bearing surfaces. J Am Acad Orthop Surg. 2009, 17: 69-76.PubMedGoogle Scholar
- Dorr LD, Bloebaum R, Emmanual J, Meldrum R: Histologic, biochemical, and ion analysis of tissue and fluids retrieved during total hip arthroplasty. Clin Orthop Relat Res. 1990, 261: 82-95.PubMedGoogle Scholar
- Michel R, Nolte M, Reich M, Loer F: Systemic effects of implanted prostheses made of cobalt-chromium alloys. Arch Orthop Trauma Surg. 1991, 110: 61-74. 10.1007/BF00393876.View ArticlePubMedGoogle Scholar
- Stulberg BN, Merritt K, Bauer TW: Metallic wear debris in metal-backed patellar failure. J Appl Biomater. 1994, 5: 9-16. 10.1002/jab.770050103.View ArticleGoogle Scholar
- Sunderman FW, Hopfer SM, Swift T, Rezuke WN, Ziebka L, Highman P, Edwards B, Folcik M, Gossling HR: Cobalt, chromium, and nickel concentrations in body fluids of patients with porous-coated knee or hip prostheses. J Orthop Res. 1989, 7: 307-315. 10.1002/jor.1100070302.View ArticlePubMedGoogle Scholar
- Medicines and Healthcare Products Regulatory Agency: Biological effects of metal wear debris generated from hip implants: genotoxicity.http://www.mhra.gov.uk/Safetyinformation/Generalsafetyinformationandadvice/Product-specificinformationandadvice/Product-specificinformationandadvice%E2%80%93M%E2%80%93T/Orthopaedicimplants/ExpertAdvisoryGrouponBiologicaleffectsofmetalweardebrisgeneratedfromhipimplantsgenotoxicity/Report/index.htm
- Tuan RS, Lee FY, Konttinen T, Wilkinson JM, Smith RL: What are the local and systemic biologic reactions and mediators to wear debris, and what host factors determine or modulate the biologic response to wear particles?. J Am Acad Orthop Surg. 2008, 16 (Suppl 1): S42-S48.PubMed CentralPubMedGoogle Scholar
- Bonewald LF: Osteocytes as dynamic multifunctional cells. Ann N Y Acad Sci. 2007, 1116: 281-290. 10.1196/annals.1402.018.View ArticlePubMedGoogle Scholar
- Aguirre JI, Plotkin LI, Stewart SA, Weinstein RS, Parfitt AM, Manolagas SC, Bellido T: Osteocyte apoptosis is induced by weightlessness in mice and precedes osteoclast recruitment and bone loss. J Bone Miner Res. 2006, 21: 605-615. 10.1359/jbmr.060107.View ArticlePubMedGoogle Scholar
- Cardoso L, Herman BC, Verborgt O, Laudier D, Majeska RJ, Schaffler MB: Osteocyte apoptosis controls activation of intracortical resorption in response to bone fatigue. J Bone Miner Res. 2009, 24: 597-605. 10.1359/jbmr.081210.PubMed CentralView ArticlePubMedGoogle Scholar
- Tatsumi S, Ishii K, Amizuka N, Li M, Kobayashi T, Kohno K, Ito M, Takeshita S, Ikeda K: Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction. Cell Metab. 2007, 5: 464-475. 10.1016/j.cmet.2007.05.001.View ArticlePubMedGoogle Scholar
- Verborgt O, Gibson GJ, Schaffler MB: Loss of osteocyte integrity in association with microdamage and bone remodeling after fatigue in vivo. J Bone Miner Res. 2000, 15: 60-67. 10.1359/jbmr.2000.15.1.60.View ArticlePubMedGoogle Scholar
- Tomkinson A, Gevers EF, Wit JM, Reeve J, Noble BS: The role of estrogen in the control of rat osteocyte apoptosis. J Bone Miner Res. 1998, 13: 1243-1250. 10.1359/jbmr.19188.8.131.523.View ArticlePubMedGoogle Scholar
- Atkins GJ, Welldon KJ, Holding CA, Haynes DR, Howie DW, Findlay DM: The induction of a catabolic phenotype in human primary osteoblasts and osteocytes by polyethylene particles. Biomaterials. 2009, 30: 3672-3681. 10.1016/j.biomaterials.2009.03.035.View ArticlePubMedGoogle Scholar
- Kanaji A, Caicedo MS, Virdi AS, Sumner DR, Hallab NJ, Sena K: Co-Cr-Mo alloy particles induce tumor necrosis factor alpha production in MLO-Y4 osteocytes: a role for osteocytes in particle-induced inflammation. Bone. 2009, 45: 528-533. 10.1016/j.bone.2009.05.020.PubMed CentralView ArticlePubMedGoogle Scholar
- Lohmann CH, Dean DD, Bonewald LF, Schwartz Z, Boyan BD: Nitric oxide and prostaglandin E2 production in response to ultra-high molecular weight polyethylene particles depends on osteoblast maturation state. J Bone Joint Surg Am. 2002, 84-A: 411-419.PubMedGoogle Scholar
- Busse B, Hahn M, Niecke M, Jobke B, Puschel K, Delling G, Katzer A: Allocation of nonbirefringent wear debris: darkfield illumination associated with PIXE microanalysis reveals cobalt deposition in mineralized bone matrix adjacent to CoCr implants. J Biomed Mater Res A. 2008, 87: 536-545. 10.1002/jbm.a.31794.View ArticlePubMedGoogle Scholar
- Caicedo M, Jacobs JJ, Reddy A, Hallab NJ: Analysis of metal ion-induced DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni2+ and V3+ are more toxic than other metals: Al3+, Be2+, Co2+, Cr3+, Cu2+, Fe3+, Mo5+, Nb5+, Zr2+. J Biomed Mater Res A. 2008, 86: 905-913. 10.1002/jbm.a.31789.View ArticlePubMedGoogle Scholar
- Hallab NJ, Anderson S, Caicedo M, Brasher A, Mikecz K, Jacobs JJ: Effects of soluble metals on human peri-implant cells. J Biomed Mater Res A. 2005, 74: 124-140. 10.1002/jbm.a.30345.View ArticlePubMedGoogle Scholar
- Kato Y, Windle JJ, Koop BA, Mundy GR, Bonewald LF: Establishment of an osteocyte-like cell line, MLO-Y4. J Bone Miner Res. 1997, 12: 2014-2023. 10.1359/jbmr.19184.108.40.2064.View ArticlePubMedGoogle Scholar
- Catelas I, Petit A, Zukor DJ, Huk OL: Cytotoxic and apoptotic effects of cobalt and chromium ions on J774 macrophages - implication of caspase-3 in the apoptotic pathway. J Mater Sci Mater Med. 2001, 12: 949-953. 10.1023/A:1012800813662.View ArticlePubMedGoogle Scholar
- Catelas I, Petit A, Zukor DJ, Antoniou J, Huk OL: TNF-alpha secretion and macrophage mortality induced by cobalt and chromium ions in vitro-qualitative analysis of apoptosis. Biomaterials. 2003, 24: 383-391. 10.1016/S0142-9612(02)00351-4.View ArticlePubMedGoogle Scholar
- Catelas I, Petit A, Vali H, Fragiskatos C, Meilleur R, Zukor DJ, Antoniou J, Huk OL: Quantitative analysis of macrophage apoptosis vs. necrosis induced by cobalt and chromium ions in vitro. Biomaterials. 2005, 26: 2441-2453. 10.1016/j.biomaterials.2004.08.004.View ArticlePubMedGoogle Scholar
- Fleury C, Petit A, Mwale F, Antoniou J, Zukor DJ, Tabrizian M, Huk OL: Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: morphology, cytotoxicity, and oxidative stress. Biomaterials. 2006, 27: 3351-3360. 10.1016/j.biomaterials.2006.01.035.View ArticlePubMedGoogle Scholar
- Hallab NJ, Vermes C, Messina C, Roebuck KA, Glant TT, Jacobs JJ: Concentration- and composition-dependent effects of metal ions on human MG-63 osteoblasts. J Biomed Mater Res. 2002, 60: 420-433. 10.1002/jbm.10106.View ArticlePubMedGoogle Scholar
- Huk OL, Catelas I, Mwale F, Antoniou J, Zukor DJ, Petit A: Induction of apoptosis and necrosis by metal ions in vitro. J Arthroplasty. 2004, 19: 84-87. 10.1016/j.arth.2004.09.011.View ArticlePubMedGoogle Scholar
- Petit A, Mwale F, Zukor DJ, Catelas I, Antoniou J, Huk OL: Effect of cobalt and chromium ions on bcl-2, bax, caspase-3, and caspase-8 expression in human U937 macrophages. Biomaterials. 2004, 25: 2013-2018. 10.1016/j.biomaterials.2003.08.040.View ArticlePubMedGoogle Scholar
- Puleo DA, Huh WW: Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cells. J Appl Biomater. 1995, 6: 109-116. 10.1002/jab.770060205.View ArticlePubMedGoogle Scholar
- Steflik DE, Sisk AL, Parr GR, Lake FT, Hanes PJ, Berkery DJ, Brewer P: Transmission electron and high-voltage electron microscopy of osteocyte cellular processes extending to the dental implant surface. J Biomed Mater Res. 1994, 28: 1095-1107. 10.1002/jbm.820280915.View ArticlePubMedGoogle Scholar
- Steflik DE, Corpe RS, Lake FT, Young TR, Sisk AL, Parr GR, Hanes PJ, Berkery DJ: Ultrastructural analyses of the attachment (bonding) zone between bone and implanted biomaterials. J Biomed Mater Res. 1998, 39: 611-620. 10.1002/(SICI)1097-4636(19980315)39:4<611::AID-JBM16>3.0.CO;2-9.View ArticlePubMedGoogle Scholar
- Noble BS: The osteocyte lineage. Arch Biochem Biophys. 2008, 473: 106-111. 10.1016/j.abb.2008.04.009.View ArticlePubMedGoogle Scholar
- Franz-Odendaal TA, Hall BK, Witten PE: Buried alive: how osteoblasts become osteocytes. Dev Dyn. 2006, 235: 176-190. 10.1002/dvdy.20603.View ArticlePubMedGoogle Scholar
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