Figure 4
From: Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
Fluorescent microscopic observation of MLO-Y4 osteocytes. Fluorescent microscopic observation of MLO-Y4 osteocytes after (A) 0.00 mM (control) or 0.50 mM (B) Co and (C) Ni ion solution treatment for 24 hr. Total cell numbers and ratio of dead cells were assessed following Hoechst 33342 and propidium iodine staining. Scale bar =100 μm.