Fig. 3

DDR2-siRNA/DDR2-blocker downregulated the gene and protein expressions of OPN, BSP, OCN and P38. A the mRNA expressions of OPN, BSP, OCN, DDR2 and P38 quantified by RT-qPCR in response to the treatment of CON, ON, Inhibitor, and DDR2-siRNA group; B the gray value of protein bands and protein levels of OPN, BSP, OCN, DDR2, P-DDR2, P38 and P-P38 quantified by western blot analysis in response to the treatment of CON, ON, Inhibitor, and DDR2-siRNA group; NS P > .05, *P < .05, **P < .01, ***P < .001 versus the Con group; #P < .05, ##P < .01, ###P < .001 versus the ON group; the experiments were conducted in triplicates; data were expressed by means ±  standard deviation (SD). OPN, osteopontin; BSP, bone sialoprotein; OCN, osteocalcin; P38: p38 mitogen‐activated protein kinase; P-P38: phosphorylated p38; DDR2: discoidin domain receptor 2; P-DDR2: phosphorylated DDR2; RT‐qPCR: reverse transcription-quantitative polymerase chain reaction; CON group: control group, the osteoblasts were added with mineralized solution; ON group: osteonectin group, the osteoblasts were added with mineralized solution + 1 µg/mL osteonectin; Inhibitor group: the osteoblasts were added with mineralized solution + 1 µg/mL osteonectin + 230 nM WRG-28; DDR2-siRNA group: the osteoblasts were transfected with DDR2-siRNA and added with mineralized solution + 1 µg/mL osteonectin