- Open Access
Review of the role of dynamic 18F-NaF PET in diagnosing and distinguishing between septic and aseptic loosening in hip prosthesis
© Adesanya et al.; licensee BioMed Central. 2015
Received: 17 September 2014
Accepted: 23 December 2014
Published: 16 January 2015
Joint replacements may fail due to infection, dislocation, peri-prosthetic fracture and loosening. Between 0.4 and 4% of joint replacements are known to be complicated by infection and aseptic loosening 2–18%. Differentiating between infection and aseptic loosening has an important bearing on the ongoing strategy for antimicrobial therapy and surgical intervention, but distinguishing one from the other can be difficult and will often require a battery of clinical and biochemical tests including the use of varying radiological modalities to accurately identify whether problematic joints are infected or aseptically loose. Prompt diagnosis is important due to the development of a biofilm on the surface of the infected prosthesis, which makes treatment difficult. There is no consensus among experts on the ideal imaging technique nor the methodology for image interpretation, but there is an increasing trend to apply hybrid imaging in the investigation of painful joint prosthesis and recent attempts have been made using PET-CT to identify aseptic loosening and infection with 18F-fluorodeoxyglucose (FDG) and sodium fluoride 18F-Na. The aim of this paper is to evaluate the role of 18F-NaF sodium fluoride (18F-NaF) positron emission tomography (PET) in distinguishing between septic and aseptic failure in hip and knee replacements, in addition to evaluating the feasibility of using multi-sequential 18F-NaF PET-CT for the assessment of painful lower limb prostheses.
Joint replacement is the surgical intervention of choice for end-stage arthritis. A small but significant proportion of these patients with joint prostheses go on to suffer implant failure. Causes for failure include infection, dislocation, peri-prosthetic fracture and loosening.
It is estimated that between 0.4 and 4% of joint replacements are known to be complicated by infection  and aseptic loosening 2–18% . Although differentiating between infection and aseptic loosening has an important bearing on the ongoing strategy for antimicrobial therapy and surgical intervention , distinguishing one from the other can be difficult and will often require a battery of clinical and biochemical tests including the use of varying radiological modalities to accurately identify whether problematic joints are infected or aseptically loose. Prompt diagnosis is important due to the development of a biofilm on the surface of the infected prosthesis, which makes treatment difficult .
There is currently no consensus among experts on the ideal imaging technique nor the methodology for image interpretation . Current imaging investigations used in problematic prosthetic joints include serial radiographs, contrast arthrography, ultrasound, magnetic resonance imaging (MRI), computed tomography (CT) and conventional Nuclear Medicine studies , supported by blood tests such as erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and the white cell count . In the absence of other inflammatory conditions, the white cell count is least useful , while CRP is most useful from the third week after joint replacement surgery, but ESR may take 3 to 12 months to return to normal levels [7,8].
CT and MRI imaging for prostheses produce high anatomical detail, but metal-related artefacts can reduce image quality and sensitivity. Ultrasound can detect soft-tissue collections; and help guide joint aspiration and capsular biopsy. In general, radionuclide imaging techniques yield little anatomical detail and are sensitive but non-specific for diagnosing loosening and infection.
Thus, there is an increasing trend to apply hybrid imaging in the investigation of painful joint prosthesis, often employing SPECT-CT, which combines the high resolution of CT and the functional sensitivity of Single Photon Emission Tomography (SPECT) . Recent attempts have been made using PET-CT to identify aseptic loosening and infection with 18F-fluorodeoxyglucose (FDG) and sodium fluoride 18F-NaF [10,11]. Sodium fluoride metabolism in bone is reliant on the rate of blood flow, which is the rate-limiting step. Most of the sodium fluoride delivered to the bone is retained after a single pass of blood  making it an excellent radiopharmaceutical to assess subtle changes in bone hyperaemia and turnover . The first pass rate varies among different bone types , with the degree NaF uptake in bone marrow being negligible when compared with the bony cortex levels . 18F-NaF bone uptake is dependent on the exchange of fluoride ions with hydroxyl ions in hydroxyapatite crystal to form fluoroapatite . 18F-NaF is freely diffusible across membranes and 1 h after injection, only 10% of 18F-NaF remains in the plasma. 18F-NaF is rapidly cleared from plasma and excreted by the renal system following glomerular filtration and tubular secretion [12,14].
The aim of this paper is to evaluate the role of 18F-NaF sodium fluoride (18F-NaF) positron emission tomography (PET) in distinguishing between septic and aseptic failure in hip and knee replacements, in addition to evaluating the feasibility of using multi-sequential 18F-NaF PET-CT for the assessment of painful lower limb prostheses.
A systematic review of the literature was undertaken according to the methods described in the Cochrane Handbook for Systematic Review of Interventions.
What is the evidence for use of sodium fluoride PET-CT in differentiating between septic and aseptic failure of hip and knee replacements?
Human prospective studies that reported data on sodium fluoride in joint prosthesis imaging to diagnose loosening and/or infection.
Studies were excluded where other isotopes other than sodium fluoride were used. Studies were limited to English language and filters for human studies and clinical trials were applied.
Studies were identified using MeSH terms and keywords in MEDLINE, EMBASE, Cochrane and Dynamed. About 133 were excluded at title and abstract; 1 was excluded at full paper review. No paper was added after review of the references.
PubMed MESH search terms -(((((((((infection) OR sepsis) OR loosening) OR aseptic loosening) OR osteolysis)) AND ((((prosthesis) OR joint replacement) OR knee replacement) OR hip replacement)) AND ((((((sodium fluoride) OR fluoride) OR fluorine) OR NaF) NOT FDG) NOT fluorodeoxyglucose))) NOT dental.
Two authors checked all data used in the analysis. When disagreements arose, these were resolved by consensus.
Initially, title and abstracts were reviewed. Potentially relevant papers were reviewed in their entirety. The references cited by each potentially relevant paper were scrutinised in order to locate additional potentially relevant papers.
The summative weighted sensitivities and specificities were calculated from the data extracted.
Summary of included studies
Asymptomatic control joints
Minimal time from surgery
Minimal clinical follow-up
Differentiate aseptic loosening from sepsis
Creutzig H. et al.
3 to 9 months
Sterner et al.
Kobayashi N et al.
Results from the included studies
Creutzig H. et al.
Sterner et al.
Kobayashi N et al.
Thomas Sterner performed the only prospective study with the use of 18F-NaF in imaging knee prosthesis, but no attempt was made to differentiate aseptic loosening from infection . Fourteen symptomatic knee prostheses were examined and no control group was employed. Of these 14 patients, 6 underwent surgery for confirmation of the imaging findings and the other 8 were followed up clinically for 6 months. The relatively low specificity in this study may have arisen from several factors including the fact that this the study included only knees, the sample size was relatively small and finally that even intermediate levels of peri-prosthetic uptake were regarded as positive for aseptic loosening or infection . He also regarded the scan as abnormal if (a) there was more uptake in the prosthesis/bone interface than in normal/bone/soft tissue or contralateral asymptomatic prosthesis, (b) the increased uptake included half the bone/metal interface in the femoral component or (c) if the tibial stem in the tibial component was involved. Furthermore, they discovered that the use of semi-quantitative analysis with standardised uptake values (SUVs) yielded no added value . Image quality in this study was bound to be better because the authors employed an ECAT-Exact HR+ (Siemens Medical Systems) PET scanner 1 and 60 min after the injection of 350 MBq of18F fluoride .
In 2011, Naomi Kobayashi et al. published a prospective study of 18F-NaF PET in 65 hip prostheses; to date, this has been the only prospective trial which differentiated aseptic loosening from sepsis . Images were acquired 40 min after injection of 185 MBq of 18F fluoride using a SET 2400W device (Shimadzu, Kyoto, Japan). She was able to distinguish between normal, aseptic loosening and infected prostheses. The first method involved measuring the degree of 18F fluoride uptake in peri-prosthetic tissues (SUVmax). Average values for the normal, aseptic and septic loosening prostheses were estimated to be 4.9 ± 2.5, 8.1 ± 2.9 and 10.5 ± 3.4, respectively . When a threshold SUVmax of 6.9 is applied for diagnosis of infection, the test had a sensitivity and specificity of 81 and 80%, respectively . Furthermore, when a threshold SUVmax of 4.9 is applied for aseptic loosening, the test yields a sensitivity and specificity of 95 and 82%, respectively .
PET images were also analysed for the pattern and distribution of 18F fluoride PET uptake and categorised into three types. Type 1 uptake showed no significant 18F fluoride uptake; type 2 uptake shows mild localised uptake on the cup side or stem. Type 3 pattern of uptake demonstrates significant uptake which extends through more than half of the bone-implant interface . In type1 pattern, 96% of the cases were normal, 80% of type 2 pattern were due to aseptic loosening and 95% of type 3 pattern cases were due to infection . The final diagnosis of infection was obtained by a combination of microbiologic culture, histopathology and polymerase chain reaction (PCR) analysis of bacterial DNA with two different primer and probe sets, one specific for the detection of methicillin-resistant staphylococcus and another for broad-range detection by universal PCR that targets a part of 16S rDNA gene, with increased sensitivity [15,20]. Many PCRs that detect the universal 16S rRNA bacterial gene have problems with false-positive results, with necrotic bacteria detected by PCRs . The clinical importance of positive results in the absence of other clinic-pathologic and radiological features of infection is of uncertain significance, but specificity can be improved by combining a universal PCR with subsequent bacterial sequencing .
It is important to distinguish between infection and loosening because the management of these conditions is very different. There is no consensus on the use of imaging algorithms for the diagnosis of loosening and infection. In general, radionuclide imaging techniques are sensitive but non-specific for diagnosing loosening and yield little anatomical detail. Furthermore, a number of radionuclide studies are often performed sequentially to improve diagnostic accuracy , further prolonging the interval between the onset of symptoms and diagnosis . Sodium fluoride positron emission tomography (18F-NaF-PET) is a promising tool with high sensitivity and specificity in the assessment of joint replacements, but it possibly will be of limited use before the ninth post-surgical month when the uptake curve ratios reach a nadir particularly in the acetabular component . This can be overcome by routinely imaging ‘at risk’ prosthesis at 3-month intervals for the detection of abnormal rates of decline in peri-prosthetic 18F-NaF uptake. The downside of this method would be its relatively high cost and high radiation dose , but with reduced scanning times with PET-CT , this may become a more attractive option. 18F-NaF-PET also avoids patient contamination as well as infection risk to technicians which may occur in labelled white cell studies. The added value of the CT component in 18F-NaF PET-CT would include the detection of anatomical lesions such as occult fractures, aseptic loosening and osteolysis as well as guiding intervention procedures .
Alternately, with fluorodeoxyglucose positron emission tomography (18F-FDG-PET), the most commonly used PET isotope, arthroplasty induces non-specific periprosthetic increased uptake which persists for several years, even in patients without evidence of infection or loosening .This can easily be misinterpreted as periprosthetic infection resulting in false positives most notably in the head and neck portions of femoral prostheses .
Serial or single sodium fluoride positron emission tomography (18F-NaF-PET) is a sensitive and specific tool for assessing joint prostheses. Furthermore, the CT component enhances its diagnostic and radiological interventional value. Wider studies are required to identify normal and pathological patterns in both hip and knee prostheses.
OA, JM, AS, JM and CH are employed or funded by University of Warwick.
- Phillips J, Crane T, Noy M, Elliott T, Grimer R. The incidence of deep prosthetic infections in a specialist orthopaedic hospital: a 15-year prospective survey. J Bone Joint Surgery-British Vol. 2006;88(7):943.View ArticleGoogle Scholar
- Berquist TH. Musculoskeletal imaging companion. Philadelphia: Lippincott Williams & Wilkins; 2006.Google Scholar
- Zoccali C, Teori G, Salducca N. The role of FDG-PET in distinguishing between septic and aseptic loosening in hip prosthesis: a review of literature. Int Orthop. 2009;33(1):1–5.PubMed CentralPubMedView ArticleGoogle Scholar
- Borens O, Tissot C, Delaloye JR, Trampuz A. Ten errors to avoid while dealing with infected total joint arthroplasties. Rev Med Suisse. 2012;8(367):2452–6.PubMedGoogle Scholar
- Gemmel F, Van den Wyngaert H, Love C, Welling MM, Gemmel P, Palestro CJ. Prosthetic joint infections: radionuclide state-of-the-art imaging. Eur J Nucl Med Mol Imaging. 2012;39(5):892–909.PubMedView ArticleGoogle Scholar
- Sofka CM. Optimizing techniques for musculoskeletal imaging of the postoperative patient. Radiol Clin North Am. 2006;44(3):323.PubMedView ArticleGoogle Scholar
- Hunter G, Dandy D. The natural history of the patient with an infected total hip replacement. J Bone Joint Surg Br. 1977;59(3):293–7.PubMedGoogle Scholar
- Toms AD, Davidson D, Masri BA, Duncan CP. The management of peri-prosthetic infection in total joint arthroplasty. J Bone Joint Surg Br. 2006;88(2):149–55.PubMedView ArticleGoogle Scholar
- Strobel K, Steurer-Dober I, Huellner MW, Veit-Haibach P, Allgayer B. Importance of SPECT/CT for knee and hip joint prostheses. Radiologe. 2012;52(7):629–35.PubMedView ArticleGoogle Scholar
- Stumpe KDM, Nötzli HP, Zanetti M, Kamel EM, Hany TF, Görres GW, et al. FDG PET for differentiation of infection and aseptic loosening in total hip replacements: comparison with conventional radiography and three-phase bone scintigraphy1. Radiology. 2004;231(2):333–41.PubMedView ArticleGoogle Scholar
- Gary JR C, Ignac F, Ora I. PET imaging of the skeleton. London: Springer London; 2013. p. 317–35. Print ISBN 978-1-85233-971-5.Google Scholar
- Czernin J, Satyamurthy N, Schiepers C. Molecular mechanisms of bone 18F-NaF deposition. J Nucl Med. 2010;51(12):1826–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Kawaguchi M, Tateishi U, Shizukuishi K, Suzuki A, Inoue T. 18F-fluoride uptake in bone metastasis: morphologic and metabolic analysis on integrated PET/CT. Ann Nucl Med. 2013;24(4):241–7.View ArticleGoogle Scholar
- Creutzig H. A comparison of osteotropic radiopharmaceuticals. II. Plasma clearance of 18F and 99mTc-EHDP (author's transl). Rofo. 1975;123(4):313–8.PubMedView ArticleGoogle Scholar
- Kobayashi N, Inaba Y, Choe H, Ike H, Fujimaki H, Tezuka T, et al. Use of F-18 fluoride PET to differentiate septic from aseptic loosening in total hip arthroplasty patients. Clin Nucl Med. 2011;36(11):E156–E61.PubMedView ArticleGoogle Scholar
- Sterner T, Pink R, Freudenberg L, Jentzen T, Quitmann H, Bockisch A, et al. The role of [18F]fluoride positron emission tomography in the early detection of aseptic loosening of total knee arthroplasty. Int J Surg. 2007;5:99–104. England.PubMedView ArticleGoogle Scholar
- Creutzig H. Bone imaging after total replacement arthroplasty of the hip joint. A follow-up with different radiopharmaceuticals. Eur J Nucl Med. 1976;1(3):177–80.PubMedView ArticleGoogle Scholar
- Blau M, Nagler W, Bender MA. Fluorine-18: a new isotope for bone scanning. J Nuclear Med. 1962;3:332–4.Google Scholar
- Blau M, Ganatra R, Bender M. 18 F-fluoride for bone imaging. Semin Nucl Med. 1972;2:31–7.PubMedView ArticleGoogle Scholar
- Levy PY, Fenollar F. The role of molecular diagnostics in implant-associated bone and joint infection. Clin Microbiol Infect. 2012;18(12):1168–75.PubMedView ArticleGoogle Scholar
- Bauer TW, Parvizi J, Kobayashi N, Krebs V. Diagnosis of periprosthetic infection. J Bone Joint Surg. 2006;88(4):869–82.PubMedView ArticleGoogle Scholar
- Love C, Tomas MB, Marwin SE, Pugliese PV, Palestro CJ. Role of nuclear medicine in diagnosis of the infected joint replacement1. Radiographics. 2001;21(5):1229–38.PubMedView ArticleGoogle Scholar
- Segura AB, Muñoz A, Brulles YR, Hermoso JAH, Díaz MC, Lazaro MTB, et al. What is the role of bone scintigraphy in the diagnosis of infected joint prostheses? Nucl Med Commun. 2004;25(5):527–32.PubMedView ArticleGoogle Scholar
- Townsend DW, Carney JP, Yap JT, Hall NC. PET/CT today and tomorrow. J Nucl Med. 2004;45(1 suppl):4S–14S.PubMedGoogle Scholar
- Zhuang H, Chacko TK, Hickeson M, Stevenson K, Feng Q, Ponzo F, et al. Persistent non-specific FDG uptake on PET imaging following hip arthroplasty. Eur J Nucl Med Mol Imaging. 2002;29(10):1328–33.PubMedView ArticleGoogle Scholar
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