Compressive loading at the end plate directly regulates flow and deformation of the basivertebral vein: an analytical study
© Yeh et al; licensee BioMed Central Ltd. 2006
Received: 11 April 2006
Accepted: 27 December 2006
Published: 27 December 2006
Metastatic diseases and infections frequently involve the spine. This is the result of seeding of the vertebral body by tumor cells or bacteria delivered by venous blood from Batson's plexus, which is hypothesized to enter the vertebral body via the epidural veins. Isolated spinal segments deform significantly at the bony end plate when under compression. This deformation could cause a volume change of the vertebral body and may be accompanied by retrograde flow of venous blood. To date, this process has not been investigated quantitatively. The purpose of this study was to determine the volume changes of the vertebral body and basivertebral vein for a vertebral body under compression.
A three-dimensional finite element mesh model of the L4 segment with both adjacent discs was modified from a 3-D computed tomography scan image. An octagon representing the basivertebral vein was introduced into the center of the vertebral body in the model. Four compressive orientations (1500 N) were applied on the top disc. The volume change of the vertebral body model and the basivertebral vein were then computed.
The volume change of the vertebral body was about 0.1 cm3 (16.3% of the basivertebral vein) for the four loading conditions. The maximum cross-sectional area reductions of the basivertebral vein and volume reduction were 1.54% and 1.02%, for uniform compression.
Our study quantified the small but significant volume change of a modeled vertebral body and cross-sectional areas and that of the basivertebral vein, due to the inward bulging of the end plate under compression. This volume change could initiate the reverse flow of blood from the epidural venous system and cause seeding of tumors or bacterial cells.
It is well-known that the venous drainage network for the bony vertebral column is unique [1–4]. The caliber of the basivertebral veins of the vertebral body is far out of proportion to similar venous drainage systems for other bones of the body, including large weight-bearing bones [1, 5]. Venous blood drains from the basivertebral vein into the epidural system via the central vascular foramen of the vertebral body. This blood then enters the highly compliant epidural system which is continuous with the valveless network of Batson's plexus .
Metastatic disease and infections frequently involve the spine. This is widely believed to be the result of seeding of the vertebral body by tumor cells or bacteria delivered to the vertebral body by venous blood from Batson's plexus, which is hypothesized to enter the vertebral body via the epidural veins [1–3, 6–9]. It has been proposed that during daily activities such as straining, coughing, or lifting with the upper extremity, blood is not only prevented from entering the thoracicoabdominal cavity, it is actually squeezed out of the cavity. Tumors and abscesses of the thoracicoabdominal viscera and retroperitoneal space connected with the Batson's plexus may therefore be shed out from the cavity and distributed anywhere along the vertebral system of veins [1, 7]. If this widely held belief is correct, then some retrograde flow of blood within the vertebral body must take place to allow intraosseus entry of bacteria or tumors. The large caliber of the basivertebral vein and the flexible epidural system therefore presents an appropriate anatomic accommodation for the volumes of venous blood which must enter and exit the vertebral body with each cycle of loading, allowing for possible intermittent retrograde flow. We speculate that large end plate deformation under normal physiological loading observed from experimental studies [10, 11] could cause a volume change of the vertebral body. This volume change is accommodated by antegrade and retrograde flow of venous blood in and out of the valveless epidural vein system, which is continuous with Batson's plexus.
Previous works have shown that isolated spinal motion segments, when subjected to axial load, deform significantly at the bony end plate [10–17]. If endplate deflection of the vertebral body is a significant mechanism for axial compression in the spine, the volume change within the vertebral bones may occur concomitantly. The spine is constantly subjected to intermittent compressive loading during daily activities. Since compressive motion of the spine occurs naturally in a rapid manner, this action requires some means for the rapid accommodation of these volume changes. This may be accomplished by the flow of venous blood. To date, the volume change of the venous vessel has not been investigated.
We hypothesize that volume within the vertebral body is directly regulated by the compressive loading applied on the end plate. The volume changes within the vertebra under compressive loading could be accommodated by the blood volume and flow of the basivertebral vein with antegrade and retrograde flow of venous blood in and out of the valveless epidural vein system. The volume change of the vertebral body directly altering the total volume of the intraosseous blood vessel could be one of the driving forces for this reverse flow of the vertebral venous system.
In this study, we examined the volume change of the vertebral body under different loading conditions using finite element analysis, and the cross-sectional area and volume changes of the basivertebral vein were calculated to verify the hypothesis.
The end plate deflection of the vertebral segment was very sensitive to the accuracy of the thickness of the cortical shell because the modulus difference between cortical and cancellous bone was 50 to 100 times. The thickness of the cortical shell from the original CT scan image might not well represent its real value. For confirming the model in this study, the end plate deflection of the vertebral segment under uniform compression was matched to the previous experimental data by deliberately adjusting the thickness of the cortical shell.
Four loading conditions were simulated with a compressive force of 1500 N applied on the top disc: uniform, lateral portion, anterior portion, and posterior portion. The bottom disc was fixed. The deformations of the end plate, vertebral body, and basivertebral vein were calculated. The change of the vertebral body volume and cross-sectional areas along the basivertebral vein and its volume were computed.
The deflection, or inward bulging, at the center of the vertebral endplate was 0.185 mm when the thickness of the bony endplates was modified from 1 mm to 0.4 mm. It was compared to the studies using experimental measurements under the same loading condition with good agreement [10, 12].
Vertebral body volume changes under different loading and Poisson's ratio (0.3, 0.1) for cancellous bones.
(ν = 0.3)
(ν = 0.1)
The cross-sectional area and volume changes at the basivertebral vein under different loading conditions.
The volume changes in the basivertebral vein were influenced by the loading conditions. The original volume of the basivertebral vein in this model was 0.62655 cm3. The volume decrements of the basivertebral vein were 1.02%, 1.14%, 0.82%, and 1.08% of the original volume of the basivertebral vein for uniform, anterior, posterior and lateral loading, respectively (Table 2).
Our study found that the volume decrease for the vertebral body was larger than 0.1 cm3, and the reduction ratio of the volume of the basivertebral vein was about 1% when the vertebral body was under 1500 N axial compression. Although the percentage volume reduction of the basivertebral vein was not large, the percentage of the vertebral body volume change to the volume of the basivertebral vein in this model could be up to 17.5% (0.11 cm3 to 0.62655 cm3) for anterior compression. The deformation of the vertebral body could be accommodated by the blood flow in and out of its basivertebral venous system. Conversely, the vertebral body and basivertebral vein would expand when the loading was removed. The cross-sectional area change of the basivertebral vein at the posterior exit, less than 0.28%, was significantly less than at the center (1.53% to 1.96%) for various loading conditions.
The deformation for posterior loading was less significant because the compression was resisted by the spinous process. This implied that it is easier to create a blood vessel volume change during spine flexion than during extension.
Previous studies have shown significant changes in vertebral body pressure according to posture and position, with increased intervertebral pressures reproduced by postures that increased axial load, a phenomenon that is not seen in weight-bearing long bones [5, 18, 19]. Axial compression at the vertebral body causing significant end plate deflection was observed by experimental measurement [10, 12]. This study further computed the vertebral body and venous blood vessel volume change under the axial loading that caused similar end plate deformation. The direct accommodation of the volume change of the basivertebral vein for the vertebral body under compression would be a flow of venous blood. The volume of the basivertebral vein only slightly changed (1%); however, the volume change of the vertebral body compared to the basivertebral vein was significant (17.5%). The volume change of the vertebral body under compression could result from the compressibility of the solid phase of cortical and cancellous bones themselves; however, the volume change could also be accompanied by intraosseous flow. The pressure applied at the end plate is over 100 times higher than intraosseous pressure. The nature of the highly porous structure of cancellous bone causes most of the accompanying deformation of the vertebral body by squeezing the space among the solid bony materials, i.e. constricting the flow of blood inside it. The volume change of the vertebral body under uniform compression was about 16.3% of the volume of the basivertebral vein; thus, almost 1/6 of the blood inside the basivertebral vein was turned over under single compressive loading. Combining the deformation of the vertebral body and the basivertebral vein, the spinous venous system would function as a sucking device to retract the blood into the spine when the compression on the vertebral body was quickly released. This result would explain the mystery of a high incidence of tumor or bacterial seeding of the spine on the basis of a pumping action on the venous blood, powered by reversible endplate deflection.
The Poisson's ratio used in the model directly affected the compressibility of the material. The volume change of the vertebral body under uniform compression for Poisson's ratio (0.3) of cancellous bone used in this model was 0.102 cm3. It increased to 0.130 cm3 when the Poisson's ratio was 0.1. The in vivo actual Poisson's ratio of the bony material is difficult to calculate because of the inclusion of the liquid phase of blood and fluid and their irrigation within the vertebral body. However, 0.3 Poisson's ratio should be a conservative estimation. The volume change of the vertebral body under uniform compression was about 16.3% of the volume of the basivertebral vein using Poisson's ratio (0.3) for cancellous bone. This percentage of volume change for vertebra under rapid loading and unloading should be the driving force to generate the retrograde flow of basivertebral blood flow.
One of the advantages of this finite element method is the ability to calculate the profile of the cross-sectional area change along the basivertebral vein. The direct assessment of the reduction of the basivertebral vein cross-sectional area by experimentation remains challenging. In a previous finite element study, the vertebral body of the two-dimensional model was assumed to be axially symmetrical, and the venous vessel was not included . In our study, the profile of the cross-sectional area change along the entire basivertebral vein was calculated, as was the volume change of the vertebral blood vessel. The deformation distribution along the blood vessel matched the bulging at the end plate. The blood vessels near the surface of the vertebral body are surrounded by a cortical bony shell, so the cross-sectional area change of the basivertebral vein at its surface exit was much less than at its center (Table 2). Although it would be easier to measure the change of the cross-sectional area of the basivertebral vein at the bony surface under loading, using this value to predict the volume change of the venous vessel would underestimate the actual volume changes within the vertebral body.
To our knowledge, this is the first theoretical simulation to elucidate the correlation between the loading at the spinal body and the high incidence of seeding of tumor cells. Limitations exist in some aspects of the model: (1) the geometry and material properties of the vertebral body and (2) the shape of the spinal venous system . The human spine is a complex biological structure which consists of alternating cortical and cancellous bony components. The elastic modulus of cortical bone is about 100 times higher than cancellous bone. The deformation of the bony segment is very sensitive to the accuracy of the dimension of the cortical end plate. The geometry in this model was obtained from a 3-D CT image; however, the thickness of the cortical bone in the original CT image might not precisely represent the true dimension of the bony end plate in individual human patients. The effects of osteoporosis on this system, for example, are not known. However, osteoporosis would be predicted to increase the deflections studied herein. The finite element model in this study was carefully evaluated before its use.
End plate deflection has been measured by cadaveric studies yielding results consistent with those of this study [10, 12]. The thickness of the cortical shell in our study was carefully adjusted from 1 mm to 0.4 mm to match the end plate deformation from previous studies.
The blood vessel was modeled using an octagonal shape inserted through the vertebral body. Although the basivertebral vein is only present at a portion of the vertebral body, the combination of the upstream small veins and capillaries might deform equivalently to a single large blood vessel inside the vertebral body. In the future, more detailed geometry representing the circulatory system inside the vertebral body and the amount of pressure change in response to the blood vessel volume change needs to be studied.
In summary, our study quantified the small but significant volume change of the vertebral body and the cross-sectional areas and volume change of the basivertebral vein due to the inward bulging of the end plate under compression. This high ratio of volume change of the vertebral body to the volume of the blood vessel could initiate reverse blood flow from the epidural venous system and cause seeding of vertebral bone tumors or bacterial cells.
- Batson OV: The function of the vertebral veins and their role in the spread of metastases. 1940. Clin Orthop. 1995, 312: 4-9.PubMedGoogle Scholar
- Crock HV, Yoshizawa H, Kame SK: Observations on the venous drainage of the human vertebral body. J Bone Joint Surg Br. 1973, 55: 528-533.PubMedGoogle Scholar
- Dommisse GF, Grobler L: Arteries and veins of the lumbar nerve roots and cauda equina. Clin Orthop. 1976, 115: 22-29.PubMedGoogle Scholar
- Crock HV, Yoshizawa H: The blood supply of the lumbar vertebral column. Clin Orthop. 1976, 115: 6-21.PubMedGoogle Scholar
- Hanai K, Kawai K, Itoh Y, Satake T, Fujiyoshi F, Abematsu N: Simultaneous measurement of intraosseous and cerebrospinal fluid pressures in lumbar region. Spine. 1985, 10: 64-68. 10.1097/00007632-198501000-00010.View ArticlePubMedGoogle Scholar
- Wiley AM, Trueta J: The vascular anatomy of the spine and its relationship to pyogenic vertebral osteomyelitis. J Bone Joint Surg Br. 1959, 41-B: 796-809.PubMedGoogle Scholar
- Oeppen RS, Tung K: Retrograde venous invasion causing vertebral metastases in renal cell carcinoma. Br J Radiol. 2001, 74: 759-761.View ArticlePubMedGoogle Scholar
- Oge HK, Aydin S, Cagavi F, Benli K: Migration of pacemaker lead into the spinal venous plexus: case report with special reference to Batson's theory of spinal metastasis. Acta Neurochir(Wien). 2001, 143: 413-416. 10.1007/s007010170098.View ArticleGoogle Scholar
- Nishijima Y, Uchida K, Koiso K, Nemoto R: Clinical significance of the vertebral vein in prostate cancer metastasis. Adv Exp Med Biol. 1992, 324: 93-100.View ArticlePubMedGoogle Scholar
- Brinckmann P, Grootenboer H: Change of disc height, radial disc bulge, and intradiscal pressure from discectomy. An in vitro investigation on human lumbar discs. Spine. 1991, 16: 641-646. 10.1097/00007632-199106000-00008.View ArticlePubMedGoogle Scholar
- Heggeness MH, Doherty BJ: Discography causes end plate deflection. Spine. 1993, 18: 1050-1053.View ArticlePubMedGoogle Scholar
- Brinckmann P, Frobin W, Hierholzer E, Horst M: Deformation of the vertebral end-plate under axial loading of the spine. Spine. 1983, 8: 851-856. 10.1097/00007632-198311000-00007.View ArticlePubMedGoogle Scholar
- Suwito W, Keller TS, Basu PK, Weisberger AM, Strauss AM, Spengler DM: Geometric and material property study of the human lumbar spine using the finite element method. J Spinal Disord. 1992, 5: 50-59.View ArticlePubMedGoogle Scholar
- McNally DS, Adams MA: Internal intervertebral disc mechanics as revealed by stress profilometry. Spine. 1992, 17: 66-73. 10.1097/00007632-199201000-00011.View ArticlePubMedGoogle Scholar
- Holmes AD, Hukins DW, Freemont AJ: End-plate displacement during compression of lumbar vertebra-disc-vertebra segments and the mechanism of failure. Spine. 1993, 18: 128-35. 10.1097/00007632-199301000-00019.View ArticlePubMedGoogle Scholar
- Knop C, Lange U, Bastian L, Oeser M, Blauth M: Biomechanical compression tests with a new implant for thoracolumbar vertebral body replacement. Eur Spine J. 2001, 10: 30-37. 10.1007/s005860000211.PubMed CentralView ArticlePubMedGoogle Scholar
- Yingling VR, McGill SM: Mechanical properties and failure mechanics of the spine under posterior shear load: observations from a porcine model. J Spinal Disord. 1999, 12: 501-508. 10.1097/00002517-199912000-00010.PubMedGoogle Scholar
- Arnoldi CC: Intraosseous hypertension. A possible cause of low back pain?. Clin Orthop. 1976, 115: 30-34.PubMedGoogle Scholar
- Esses SI, Moro JK: Intraosseous vertebral body pressures. Spine. 1992, 17: S155-S159. 10.1097/00007632-199206001-00016.View ArticlePubMedGoogle Scholar
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