Fig. 4

Circ_0076684 acts as a miRNA sponge to raise CUX1 expression. A Subcellular distribution of circ_0076684 detected by RNA FISH assay. B MiRNAs potentially binding to circ_0076684 predicted by Starbase database. C The interaction between circ_0076684 and miR-370-3p, miR-140-3p or miR-193a-3p validated by RNA pull-down assay. D The predicted binding sites of miR-370-3p, miR-140-3p and miR-193a-3p on circ_0076684 verified by dual-luciferase reporter assays. E Potentially binding sites of miR-370-3p, miR-140-3p and miR-193a-3p on CUX1 3′UTR predicted by Starbase database. F The predicted binding sites of miR-370-3p, miR-140-3p and miR-193a-3p on CUX1 3′UTR verified by dual-luciferase reporter assays. G, H Regulation of miR-370-3p, miR-140-3p and miR-193a-5p on CUX1 protein expression analyzed by western blot assays. I, J Regulation of circ_0076684 on CUX1 protein expression by miR-370-3p, miR-140-3p and miR-193a-5p analyzed by western blot assays. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001