Fig. 3

Regulation of circPRKD3 affected osteogenesis of mechanically stimulated PDLSCs. (A) Schematic diagram of virus construction. Immunofluorescence staining (B) and qRT-PCR verification (C) of transfection efficiency in sh-NC and sh-circPRKD3 groups. (D-E) The mRNA expression level of ALP and RUNX2 in mechanically stimulated sh-NC and sh-circPRKD3 groups after transfection for 24 h. (F-H) The protein level of ALP and RUNX2 and the quantitative data analyzed by image J in mechanically stimulated sh-NC groups and sh-circPRKD3 groups after transfection for 24 h. Full-length blots were presented in Supplementary Fig. 2 of additional file 1. (I) Schematic diagram of plasmids construction. (J) The head-to‐tail splicing of circPRKD3 as a qRT‐PCR product was verified by Sanger sequencing. (K) Pure E. coli containing circPRKD3-overexpressed plasmids were filtered out by ampicillin. Verification of transfection efficiency in 293T (L) and PDLSCs (M) was assayed by qRT‐PCR. (N-O) The mRNA expression level of ALP and RUNX2 in mechanically stimulated pLC5‐ciR-NC groups and pLC5‐circPRKD3 groups after transfection for 24 h. (P-R) The protein level of ALP and RUNX2 and the quantitative data analyzed by image J in mechanically stimulated pLC5‐ciR-NC groups and pLC5‐circPRKD3 groups after transfection for 24 h. Full-length blots were presented in Supplementary Fig. 3 of additional file 1. Data are presented as mean ± SD of three independent experiments. (n.s., no significance; **p < 0.01; ****p < 0.0001)