Table 1 Primer sequences of genes
Gene name | Base sequence 5′-3′ | Length |
|---|---|---|
Collagen II | F: GTAACCCTGGAACAGATGGAAT | 149 |
| Â | R: TTCACCCGTCTGACCTTTCG | Â |
Collagen X | F: ACAGGCAACAGCATTATGACCC | 198 |
| Â | R: CGATGATGGCACTCCCTGAA | Â |
Aggrecan | F: ATTTCAGCGGTTCCTTCTCCA | 212 |
| Â | R: GTATAGGCTGGTTCCCATTCTG | Â |
MMP-13 | F: AATGCAGTCTTTCTTCGGCTTAG | 188 |
| Â | R: CAGAATGAGTCATATCAGGGGTGT | Â |
ADAMTS 5 | F: GAGCCTGGAAGTGAGCAAGAA | 137 |
| Â | R: CACATAAATCCTCCCGAGTAAACA | Â |
IL-1β | F: CGATCACTGAACTGCACGCTC | 131 |
| Â | R: ACAAAGGACATGGAGAACACCACTT | Â |
COX2 | F: AGCACTTCACGCATCAGTTTTTC | 205 |
| Â | R: GCCTGAGTATCTTTGACTGTGGG | Â |
GADPH | F: GGAAGCTTGTCATCAATGGAAATC | 168 |
| Â | R: TGATGACCCTTTTGGCTCCC | Â |