Fig. 5

miR-150-5p blocked the p38-MAPK pathway by inhibiting FNDC5. After 15 days of osteogenic differentiation induction of mouse BMSCs transfected with different transfection, the expression of p38 and p-p38 protein was assessed by western blot and the relative grayscale values of p38, p-p38, and p-p38/p38 ratio were measured. *** P < 0.001 vs. Control group using unpaired t test. ## P < 0.01 and ### P < 0.001 vs. Mimic group. Mimic, stable overexpression of miR-150-5p. OE-FNDC5, table overexpression of FNDC5. Mimic + OE-FNDC5, co-transfection of plasmid overexpressing FNDC5 and plasmid overexpressing miR-150-5p