Fig. 4

miR-150-5p inhibited osteogenic differentiation of BMSCs by targeting FNDC5. (A) Detection of FNDC5 protein expression levels in mouse BMSCs transfected with Mimic and/or OE-FNDC5 using western blot. (B-D) After 15 days of osteogenic differentiation induction in different cell lines of mouse BMSCs, we determined osteocalcin content (B), ALP activity (C), and calcium deposition levels (D). (E-H) Detection and statistical analysis of BMP2, OCN, and Runx2 protein levels in transfected BMSC by western blot. *** P < 0.001 vs. Control group using unpaired t test. # P < 0.05, ## P < 0.01 and ### P < 0.001 vs. Mimic group. Mimic, stable overexpression of miR-150-5p. OE-FNDC5, table overexpression of FNDC5. Mimic + OE-FNDC5, co-transfection of plasmid overexpressing FNDC5 and plasmid overexpressing miR-150-5p