Fig. 3

miR-150-5p targeted inhibits FNDC5 expression. (A) Predicted binding location of human (up) or mouse (down) miR-150-5p to the FNDC5 gene using bioinformatics in TargetScanHuman (https://www.targetscan.org/vert_71/). (B) Luciferase reporter containing WT or MUT FNDC5 sequences were transfected into mouse BMSCs prior to transfect with miR-150-5p mixed transfection reagent, and harvested the activity of luciferase. (C-D) Detection of FNDC5 mRNA (C) and protein (D) expression levels in mouse BMSCs with different transfection using RT-qPCR and western blot, respectively. *** P < 0.001 vs. Control group using unpaired t test. miR-NC, transfection negative control. Mimic, stable overexpression of miR-150-5p. Inhibitor, stable knocking down miR-150-5p