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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: mir-150-5p inhibits the osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting irisin to regulate the p38/MAPK signaling pathway

Fig. 2

miR-150-5p inhibited osteogenic genes expression. (A-F) After 15 days of osteogenic differentiation induction, we determined the expression of Runx2 (A), OSX (B), OCN (C), OPN (D), ALP (E) and BMP2 (F) mRNA using RT-qPCR. (G) After 15 days of osteogenic differentiation induction, we determined the expression of BMP2, OCN and Runx2 protein using western blot. Statistical analysis of grayscale values of BMP2, OCN, and Runx2 protein bands. *** P < 0.001 vs. Control group using unpaired t test. miR-NC, transfection negative control. Mimic, stable overexpression of miR-150-5p. Inhibitor, stable knocking down miR-150-5p

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X