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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: mir-150-5p inhibits the osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting irisin to regulate the p38/MAPK signaling pathway

Fig. 1

miR-150-5p inhibited the cell proliferation and osteogenic differentiation of mouse BMSCs. (A) Morphology of third-generation mouse BMSCs. (B) Identification of cell surface markers (CD29, CD34, CD45, CD90) in isolated mouse BMSCs using flow cytometry. (C) Detection of miR-150-5p expression levels in different cell lines of mouse BMSCs using RT-qPCR method. (D) Determination of cell viability in BMSCs transfected with Mimic or Inhibitor using CCK-8 assay kit. (E-G) After 15 days of osteogenic differentiation induction, osteocalcin content (D), ALP activity (E), and calcium deposition levels (F) were measured used a reagent kit. *** P < 0.001 vs. Control group using unpaired t test. miR-NC, transfection negative control. Mimic, stable overexpression of miR-150-5p. Inhibitor, stable knocking down miR-150-5p

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X