Fig. 3

Inhibition of osteogenic differentiation of rat BMSCs by p75NTR. (A) Measurement of cell osteogenic differentiation ability using ARS assay (Magnification: ×100, scale bar = 100 μm). (B) RT-qPCR detection of gene expression related to osteogenesis. (C) WB analysis was used to detect the expression of genes associated with osteogenesis. (D) IF double-staining was performed to assess the level of p75NTR and sortilin proteins in differentiating osteoblasts (Magnification: ×400, scale bar = 25 μm). *P < 0.05 vs. si-NC. #P < 0.05 vs. oe-NC