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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Silencing p75NTR regulates osteogenic differentiation and angiogenesis of BMSCs to enhance bone healing in fractured rats

Fig. 1

Isolation and identification of rat BMSCs. (A) Observation of cell morphology (Magnification: ×100, scale bar = 100 μm; ×200, scale bar = 50 μm). (B) FCM analysis to determine the cell cycle of the isolated cell population. *P < 0.05 vs. G0/G1. (C) CCK-8 analysis to determine the cell viability of the isolated cell population. *P < 0.05 vs. day 2, #P < 0.05 vs. day 5, $P < 0.05 vs. day 7, &P < 0.05 vs. day 9. (D) Observation of ARS for cell osteogenic differentiation (Magnification: ×100, scale bar = 100 μm). (E) Observation of Oil Red O staining for cell adipogenic differentiation (Magnification: ×400, scale bar = 25 μm). (F) FCM analysis of surface markers on BMSCs

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X