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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Inhibition of cc chemokine receptor 10 ameliorates osteoarthritis via inhibition of the phosphoinositide-3-kinase/Akt/mammalian target of rapamycin pathway

Fig. 6

Effects of CCR10-siRNA or 740Y-P on IL-1β-induced cell viability, apoptosis, and inflammatory cytokines secretion. The CHON-001 cells were divided into three groups: IL-1β + control-siRNA, IL-1β + CCR10-siRNA, and IL-1β + CCR10-siRNA + 740Y-P groups. (A) MTT assay for cell viability. (B) Determination of LDH level. (C) Apoptotic cells were evaluated using flow cytometry. (D) Quantification of the apoptotic cells. (E) Determination of cleaved-caspase-3 expression. (F) Quantization of cleaved-caspase-3 expression. The levels of TNF-α (G), IL-6 (H), and IL-8 (I) were analyzed using ELISA. (J) Western blot analysis of MMP-3, MMP-13, Collagen II, and Aggrecan. (K) MMP-3/β-actin ratio. (L) MMP-13/β-actin ratio. (M) Collagen II /β-actin ratio. (N) Aggrecan/β-actin ratio. **P < 0.01

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X