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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Inhibition of cc chemokine receptor 10 ameliorates osteoarthritis via inhibition of the phosphoinositide-3-kinase/Akt/mammalian target of rapamycin pathway

Fig. 3

Effects of CCR10-siRNA on IL-1β-induced cell viability, apoptosis and inflammatory cytokines secretion. The CHON-001 cells were divided into four groups: control, IL-1β, IL-1β + control-siRNA, or IL-1β + CCR10-siRNA group. (A) Cell viability was assessed using MTT assay. (B) Analysis of LDH release. (C) Apoptosis was assessed by flow cytometry. (D) Quantification of apoptotic CHON-001 cells. (E) Western blot analysis of cleaved-caspase-3 expression. (F) Relatively cleaved-caspase-3 protein expression were quantified. The secretion of TNF-α (G), IL-6 (H) and IL-8 (I) were evaluated by ELISA. (J) Western blot analysis of MMP-3, MMP-13, Collagen II, and Aggrecan. (K) MMP-3/β-actin ratio. (L) MMP-13/β-actin ratio. (M) Collagen II /β-actin ratio. (N) Aggrecan/β-actin ratio. **P < 0.01

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X