Fig. 6

MiR-214-3p inhibited IL-1β-induced inflammatory damage in chondrocytes. CHON-001 cells were stimulated by 10 ng/ml IL-1β for 12 h and transfected with mimic control or miR-214-3p mimic. A, B measurement of the miR-214-3p level using qRT-PCR. C MTT assay of cell viability. D LDH release was measured to evaluate cell injury. E Cellular apoptosis was evaluated by FCM. F Quantification of apoptotic cells. G Western blotting analysis of BAX and BCL2 protein expression. qRT-PCR analysis of BAX (H) and BCL2 (I) mRNA expression in the different groups. The release of IL-6 (J), IL-8 (K) and TNF-α (L) was quantified using ELISA. *P < 0.05, **P < 0.01 versus control group; ^##P < 0.01 versus IL-1β + mimic control group