Table 1 Primers used for quantitative RT-PCR
From: Iron accumulation induced by hepcidin1 knockout accelerates the progression of aging osteoporosis
Gene | Primers (Forward/Reverse) |
|---|---|
β-actin | 5′-AGATGTGGATCAGCAAGCAG-3′ 5′-GCGCAAGTTAGGTTTTGTCA-3′ |
OCN | 5′-GGACCATCTTTCTGCTCACTCTG-3′ 5′-GTTCACTACCTTATTGCCCTCCTG-3′ |
ALP | 5′-CCAACTCTTTTGTGCCAGAGA-3′ 5′-GGCTACATTGGTGTTGAGCTTTT-3′ |
Runx2 | 5′-AACTTCCTGTGCTCCGTGCTG-3′ 5′-TCGTTGAACCTGGCTACTTGG-3′ |
SP7 | 5′-AGGAGGCACAAAGAAGCCATAC-3′ 5′-GATGCCTGCCTTGTACCACGAGC-3′ |
Col-1 | 5′-ACGTCCTGGTGAAGTTGGTC-3′ 5′-CAGGGAAGCCTCTTTCTCCT-3′ |
RANKL | 5′-CACCATCAGCTGAAGATAGT-3′ |
5′-CCAAGATCTCTAACATGACG-3′ | |
OPG | 5′-AGTCCGTGAAGCAGGAGTG-3′ |
5′-CCATCTGGACATTTTTTGCAAA-3′ | |
Trap | 5′-TACCTGTGTGGACATGACC-3′ |
5′-CAGATCCATAGTGAAACCGC-3′ | |
Mmp9 | 5′-TCCAGTACCAAGACAAAG-3′ |
5′-TTGCACTGCACGGTTGAA-3′ | |
CTSK | 5′-GCCGTGGCGTTATACATACA-3′ |
5′-CTTCCAATACGTGCAGCAGA-3′ |