Fig. 6

A The cell viability of mBMSCs treated with different concentrations of D-galactose (0–100 g/L) for 24 h was measured by CCK-8. B The transfection efficiency of hepcidin1 in mBMSCs was detected by real-time quantitative PCR. C The effect of hepcidin1 on the viability of mBMSCs determined by CCK-8. D Apoptosis of mBMSCs was detected by flow cytometry. E The changes in calcium nodules after transfection of mBMSCs were evaluated by Alizarin red staining (Amplification: 200× , Scale: 100 μm). F The proliferation of mBMSCs was detected by EdU assay. G The concentration of iron ions in mBMSCs was detected by Iron Colorimetric Assay Kit. H The expression level of ferritin protein was detected by western blot. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. Control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. D-gal + siNC group