Fig. 8

Circ_0006640 transferred by MSC-exosomes protected LPS-induced microglial cell injury. (A-N) BV2 cells were incubated with PBS, Exo-vector or Exo-oe-circ_0006640, followed by LPS treatment. A qRT-PCR for miR-382-5p expression. B Western blotting analysis for IGF1 expression. C CCK-8 for cell viability analysis. D Flow cytometry for cell apoptosis analysis. E–H Western blotting analysis for the levels of Bax, Bcl-2 and cleaved caspase-3. I-K ELISA analysis for TNF-α, IL-1β, and IL-10 level. (L-N) Detection of ROS, CAT and SOD levels using commercial kits. *P < 0.05