Fig. 6

MiR-382-5p inhibition suppressed apoptosis, inflammation and oxidative stress in LPS-induced microglial cells by IGF1. (A-N) BV2 cells were transfected with In-miR-382-5p alone or co-transfected with In-miR-382-5p and si-IGF1, followed by LPS treatment. A Western blotting analysis for IGF1 expression. B CCK-8 for cell viability analysis. C, D Flow cytometry for cell apoptosis analysis. E–H Western blotting analysis for the levels of Bax, Bcl-2 and cleaved caspase-3. I-K ELISA analysis for TNF-α, IL-1β, and IL-10 level. L-N Detection of ROS, CAT and SOD levels using commercial kits. *P < 0.05