Fig. 5

IGF1 is a target of miR-382-5p. A The binding sites of miR-382-5p on IGF1. B Dual-luciferase reporter assay was used to evaluate the luciferase activity in BV2 cells transfected with recombinant vectors and miR-382-5p or miR-NC. C The interference efficiency of In-miR-382-5p or In-miR-NC was determined by qRT-PCR. D Detection of IGF1 levels in BV2 cells after miR-382-5p overexpression or underexpression by western blotting. E–G qRT-PCR and western blotting analysis of IGF1 expression in SCI mice model and LPS-induced BV2 cells. H Detection of the effects of circ_0006640/miR-382-5p axis on IGF1 expression in BV2 cells by western blotting. *P < 0.05