Fig. 4

Circ_0006640 protected microglial cells from LPS-induced apoptotic, inflammatory and oxidative injury via miR-382-5p. (A-N) BV2 cells were transfected with oe-circ_0006640 alone or co-transfected with oe-circ_0006640 and miR-382-5p, followed by LPS treatment. A qRT-PCR analysis for miR-382-5p expression. B CCK-8 for cell viability analysis. C, D Flow cytometry for cell apoptosis analysis. E–H Western blotting analysis for the levels of Bax, Bcl-2 and cleaved caspase-3. I-K ELISA analysis for TNF-α, IL-1β, and IL-10 level. L-N Detection of ROS, CAT and SOD levels using commercial kits. *P < 0.05