Fig. 2

Circ_0006640 overexpression suppressed apoptosis, inflammation and oxidative stress in LPS-induced microglial cells. (A-N) BV2 cells were transfected with oe-circ_0006640 or vector, followed by LPS treatment. A qRT-PCR analysis for circ_0006640 expression. B CCK-8 for cell viability analysis. C, D Flow cytometry for cell apoptosis analysis. E–H Western blotting analysis for the levels of Bax, Bcl-2 and cleaved caspase-3. I-K ELISA analysis for TNF-α, IL-1β, and IL-10 level. L-N Detection of ROS, CAT and SOD levels using commercial kits. *P < 0.05