Fig. 8

Effects of sh-circ_0000376 on OS tumor growth. A Circ_0000376 expression was detected by qRT-PCR in U2OS cells transfected with sh-NC or sh-circ_0000376. B–G U2OS cells transfected with sh-NC or sh-circ_0000376 were injected into nude mice. Tumor volume (B) and weight (C) were measured. D QRT-PCR was used to detect circ_0000376 and miR-577 expression. E, F WB analysis was performed to determine HK2 and LDHA protein levels. G IHC staining was used to determine HK2, LDHA and Ki67 positive cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001