Fig. 7

Effects of miR-577 and LDHA on OS cell progression. MG63 and U2OS cells were transfected with miR-577 mimic and pcDNA LDHA overexpression vector. A The LDHA protein expression was tested using WB analysis. Cell proliferation, apoptosis and invasion were analyzed by CCK8 assay (B), colony formation assay (C), EDU assay (D), flow cytometry (E) and transwell assay (F). G–I Glucose consumption, lactate production and ATP/ADP ratio were examined to evaluate cell glycolysis. J–K The protein levels of CyclinD1 and MMP9 were analyzed using WB analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001