Fig. 6

Effects of miR-577 and HK2 on OS cell progression. MG63 and U2OS cells were transfected with miR-577 mimic and pcDNA HK2 overexpression vector. A HK2 protein expression was detected by WB analysis. CCK8 assay (B), colony formation assay (C), EDU assay (D), flow cytometry (E) and transwell assay (F) were employed to analyze cell proliferation, apoptosis and invasion. G–I Glucose consumption, lactate production and ATP/ADP ratio were analyzed to measure cell glycolysis. J–K WB analysis was performed to detect CyclinD1 and MMP9 protein levels. **P < 0.01, ***P < 0.001, ****P < 0.0001