Fig. 5

MiR-577 targeted HK2 and LDHA. A, B The binding sites between miR-577 and HK2 3’UTR or LDHA 3’UTR were exhibited. C, D Dual-luciferase reporter assay was used to confirm the interaction between miR-577 and HK2 or LDHA. E HK2 mRNA expression in 33 paired OS tumor tissues and adjacent normal tissues was determined by qRT-PCR. F Pearson correlation analysis was performed. G QRT-PCR was used to detect LDHA mRNA expression in 33 paired OS tumor tissues and adjacent normal tissues. H Pearson correlation analysis was used. I–L HK2 and LDHA protein expression levels in OS tissues and cells were detected by WB analysis. **P < 0.01, ***P < 0.001, ****P < 0.0001