Fig. 4

Effects of si-circ_0000376 and anti-miR-577 on OS cell progression. MG63 and U2OS cells were transfected with si-circ_0000376 and anti-miR-577. A The miR-577 expression was detected by qRT-PCR. Cell proliferation, apoptosis and invasion were determined using CCK8 assay (B), colony formation assay (C), EDU assay (D), flow cytometry (E) and transwell assay (F). G–I Cell glycolysis was assessed by glucose consumption, lactate production and ATP/ADP ratio. J, K WB analysis was used to measure the protein levels of CyclinD1 and MMP9. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001