Fig. 3

Circ_0000376 sponged miR-577. A Venn diagram showed the miRNA predicted by starbase software and circinteractome software together. B The binding sites between circ_0000376 and miR-577 were exhibited. C The transfection efficiency of miR-577 mimic was assessed by qRT-PCR. D, E Dual-luciferase reporter assay was used to confirm the interaction between circ_0000376 and miR-577. F MiR-577 expression was examined by qRT-PCR in 33 paired OS tumor tissues and adjacent normal tissues. G Pearson correlation analysis was used. H MiR-577 expression was detected by qRT-PCR in OS cells and hFOB1.19 cells. **P < 0.01, ***P < 0.001, ****P < 0.0001