Fig. 2

The overexpression of piRNA hsa_piR_019949 has been found to promote the anabolic metabolism and inhibited catabolic of chondrocytes. A The expression of hsa_piR_019949 in chondrocytes transfected with hsa_piR_019949 mimics is being studied. B The detection of chondrocytes transfected with hsa_piR_019949 mimics was accomplished using CCK-8 assays. C The ability of chondrocytes transfected with hsa_piR_019949 mimics to form clones was detected using crystal violet staining. D Quantitative analysis of the clone formation number of chondrocytes. E The flow cytometry technique was employed to detect the apoptosis of chondrocytes that were transfected with hsa_piR_019949 mimics. F Quantitative analysis of the normal cells of chondrocyte detected by flow cytometry. (G) Safranine O was used to quantify the cartilage extracellular matrix. The protein expression of collagen II (H) and MMP13 (J) in chondrocytes transfected with hsa_piR_019949 mimics was detected through immunofluorescence staining. The mRNA expression of collagen II (I) and MMP13 (K) in chondrocytes transfected with hsa_piR_019949 mimics was detected using RT-qPCR. Statistically significant differences are indicated by *P < 0.05, **P < 0.01. n = 3/group