Fig. 4

c-Myc acted as a m⁶A-modified target in OS. A In silico tools analysis (http://gepia.cancer-pku.cn/index.html) showed the up-regulated level in the osteosarcoma (SARC) samples. B RT-PCR was performed to test the level of c-Myc in DOX-resistant OS cells (Saos-2/Dox, MG63/Dox) and parental cells (Saos-2, MG63). C In silico predictive analysis (http://www.cuilab.cn/sramp)showed that there were several potential m⁶A modified sites in the c-Myc gene. D m⁶A motif predicted by in silico tools for c-Myc gene (GGAC). E The accurate loci on c-Myc gene (GGAC). Data were presented as means ± SD from three independent experiments. **p < 0.01