Fig. 3

circARHGAP12 promoted the aerobic glycolysis of OS cells. A The glucose uptake level analysis was performed to detect the glucose uptake of Saos-2/Dox cells with circARHGAP12 silencing, and MG63/Dox cells with circARHGAP12 overexpression. B Lactate production was performed to detect the lactate level of Saos-2/Dox cells with circARHGAP12 silencing, and MG63/Dox cells with circARHGAP12 overexpression. C ATP analysis was performed to detect the ATP level of Saos-2/Dox cells with circARHGAP12 silencing, and MG63/Dox cells with circARHGAP12 overexpression. D, E Metabolic flux analysis was performed to detect the glycolysis rate of Saos-2/Dox and MG63/Dox cells with circARHGAP12 overexpression/silencing for extracellular acidification rate (ECAR). Data were presented as means ± SD from three independent experiments. *p < 0.05