Fig. 2

circARHGAP12 accelerated the Dox-resistance and proliferation of OS cells. A RT-PCR assay was performed to detect the circARHGAP12 level in DOX-resistant OS cells (Saos-2/Dox, MG63/Dox) and parental cells (Saos-2, MG63). B The silencing and overexpression of circARHGAP12 were constructed and, respectively, transfected into Dox-resistant OS cells (Saos-2/Dox, MG63/Dox) for gain/loss function assays. C, D The Dox resistance of OS cells (Saos-2/Dox, MG63/Dox) was determined by IC50 value (50% maximal inhibitory concentration) using CCK-8 assay. E, F Clone formation assay was performed to detect the colony number of Saos-2/Dox cells with circARHGAP12 silencing, and MG63/Dox cells with circARHGAP12 overexpression. Data were presented as means ± SD from three independent experiments. **p < 0.01, *p < 0.05