Fig. 1

circARHGAP12 was a DOX-resistant circRNA in OS. A CircRNA microarray was performed to test the circRNA profile in the DOX-resistant OS cells (MG63/Dox) and parental cells (MG63). B RT-PCR was performed to test several potential circRNAs (circHIPK3, circBACH2, Cdr1as, circPVT1, circARHGAP12) in the DOX-resistant OS cells (MG63/Dox) and parental cells (MG63). C Schematic diagram illustrated the biogenesis of circARHGAP12 (circARHGAP12, hsa_circ_0000231) generated from the exon-3 to exon-2 (794 bp length) of ARHGAP12 gene. D RNase R assay was performed to detect the stability of circARHGAP12 in DOX-resistant OS cells (MG63/Dox), comparing with liner transcript ARHGAP12 mRNA. E Actinomycin D assay was performed to detect the stability of circARHGAP12 in DOX-resistant OS cells (MG63/Dox). (F) RT-PCR was performed to detect the circARHGAP12 in OS cells (Saos-2, MG63) compared to normal cells (hFOB). Data were presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01