Table 1 qPCR primers
Forward primer (5′–3′) | Reverse primer (5′–3′) | |
|---|---|---|
Circ_HECW2 | CCCACCACTTTGAACGCTAC | GGCTGTCAATGCGTGCCT |
HECW2 | TACCACGGCATTAGTGGAGC | ATCCCTTTCTTTAGCCCAACTG |
pre-miR-1224 | TCGGCAGGGTGAGGACTCGGG | CAGCCACAAAAGAGCACAAT |
miR-1224-5p | GCCGAGGTGAGGACTCGGGAGG | CTCAACTGGTGTCGTGGA |
PDK2 | CCCCGTCCCCGTTGTC | TCGCAGGCATTGCTGGAT |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
GAPDH | CGCTCTCTGCTCCTCCTGTTC | ATCCGTTGACTCCGACCTTCAC |