Fig. 6

PDK2 was a downstream target gene of miR-1224-5p and was highly expressed in OP patients. A The downstream target genes of miR-1224-5p were predicted through the TargetScan7.2 and miRDB databases. B The binding site of miR-1224-5p and PDK2 was predicted through the TargetScan7.2 database. C Dual-luciferase reporter gene experiment determined the binding relationship between miR-1224-5p and PDK2. D RT-qPCR detected PDK2 mRNA expression in serum. E Correlation analysis between serum miR-1224-5p and PDK2. F RT-qPCR detected PDK2 mRNA expression in cells. The cell experiments in panels C/F were independently repeated 3 times. D Control, n = 65; OP, n = 65. Data in panel D were analyzed using independent t test. Data in panel C were analyzed using two-way ANOVA, and data in panel F were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test, *P < 0.05