Fig. 5

miR-1224-5p inhibition reversed the inhibitory effect of HECW2 downregulation on osteoblast apoptosis. Before LPS treatment, HFOB1.19 cells were transfected with anti-miR-1224-5p, with anti-NC as the control, followed by combined treatment with si-Circ_HECW2. A RT-qPCR detected miR-1224-5p expression in cells. B TUNEL detected cell apoptosis. C Western blot detected the apoptosis related protein cleaved-caspase-3. The cell experiments were independently repeated 3 times. Data were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test, *P < 0.05