Fig. 4

Circ_HECW2 reduced the formation of mature miR-1224-5p by binding to pre-miR-1224. A Subcellular fractionation experiment determined Circ_HECW2 distribution in HFOB1.19 cells. B The binding site between Circ_HECW2 and pre-miR-1224 was predicted on the IntaRNA website. C Dual-luciferase reporter gene experiment determined the binding relationship between Circ_HECW2 and pre-miR-1224. D RNA pull-down detected the enrichment of Circ_HECW2 on the binding site of pre-miR-1224. E RT-qPCR detected pre-miR-1224 expression in serum. F Correlation analysis between serum Circ_HECW2 and pre-miR-1224 in OP patients. G RT-qPCR detected pre-miR-1224 expression in HFOB1.19 cells. H RT-qPCR detected miR-1224-5p expression in serum. I Correlation analysis between serum Circ_HECW2 and miR-1224-5p in OP patients. J RT-qPCR detected miR-1224-5p expression in HFOB1.19 cells. The cell experiments in panels A/C/D/G/J were independently repeated 3 times. E, H Control, n = 65; OP, n = 65. Data in panels E/H were analyzed using independent t test. Data in panel C were analyzed using two-way ANOVA, and data in panels D/G/J were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test, *P < 0.05