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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Mechanism of Circ_HECW2 regulating osteoblast apoptosis in osteoporosis by attenuating the maturation of miR-1224-5p

Fig. 3

Downregulation of Circ_HECW2 suppressed LPS-induced osteoblast apoptosis. Before LPS treatment, HFOB1.19 cells were transfected with si-Circ_HECW2, with si-NC as the control. A-B: RNase R enzyme digestion experiment (A) and actinomycin D experiment (B) determined the circular structure of Circ_HECW2. C RT-qPCR detected Circ_HECW2 expression in cells. D TUNEL detected cell apoptosis. E Western blot detected the apoptosis related protein cleaved-caspase-3. The cell experiments were independently repeated 3 times. Data in panels AB were analyzed using two-way ANOVA, and data in panels C/D/E were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test, *P < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X