Fig. 4

CS attenuates OS cell migration and invasiveness by downregulating CXCR4. Saos-2 and U2OS were transfected with oe-CXCR4, and then treated with 200 μg/mL CS for 24 h. RT-qPCR (A) and Western blotting (B) quantified CXCR4 mRNA and protein expression, respectively. C, D Transwell assay tested cell migration and invasiveness. N = 3; the results are expressed as the mean ± standard deviation. Compared with oe-NC group, ^aP < 0.05; compared with oe-NC + CS group, ^bP < 0.05; compared with oe-CXCR4 + CS group, ^cP < 0.05. CS: Caulis Spatholobi