Fig. 3
CXCR4 knockout suppresses OS cell migration and invasiveness. RT-qPCR A and B Western blotting determined CXCR4 expression in Saos-2 and U2OS treated with AMD3100 or transfected with si-CXCR4. (C) MTT assay detected Saos-2 and U2OS cell viability after AMD3100 treatment or si-CXCR4 transfection. D–E Transwell analysis was performed to observe the migration and invasiveness of Saos-2 and U2OS treated with AMD3100 or transfected with si-CXCR4. N = 3; the results are represented by the mean ± standard deviation. In comparison with the control group or si-NC group, aP < 0.05. CS, Caulis Spatholobi