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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Long non-coding RNA KCNQ10T1/miR-19a-3p/SMAD5 axis promotes osteogenic differentiation of mouse bone mesenchymal stem cells

Fig. 5

Silenced Smad5 inhibited the role of downregulated miR-19-3p in BMSCs. Knockout efficiency of miR-19a-3p inhibitor (A) and si-Smad5 (B) was detected by RT-qPCR assay. Then the BMSCs were cultured in osteogenic medium and transfected with miR-19a-3p inhibitor and si-Smad5, C the cell viability was detected by CCK-8 assay. The positive mineralized nodules were stained by ALP (D) as well as Alizarin Red (E). F The apoptosis rate was measured by flow cytometry. The mRNA (G) and protein (H) expressions of Smad5, ALP, OPG, OCN and OPN were detected by RT-qPCR and western blot assays. **P < 0.01

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X