Fig. 5

Expression characteristics of miR-96-5p in IDD. A qRT-PCR revealed the concentration of miR-96-5p in HNPCs after incubation with indicated concentrations of IL-1β for 48 h. B qRT-PCR was used to confirm the value of miR-96-5p in HNPCs after incubation with 10 mg/mL concentration of IL-1β for various times. C The expression of miR-96-5p was assessed by qRT-PCR in degenerated IVD NP tissues of a mild IDD group and severe IDD group. D, E Western blot assay was used to analyze the protein expression of PPARγ in degenerated IVD NP tissues of a mild IDD group and severe IDD group. F, G Western blot assay was used to analyze the protein expression of NF-κB in the cytoplasm and nucleus of degenerated IVD NP tissues in a mild IDD group and a severe IDD group. (*P < 0.05, **P < 0.01, ***P < 0.001 vs. previous nearby concentration group/time point group/Mild IDD group). IL-1β, interleukin-1β; IDD, intervertebral disc degeneration; PPARγ, peroxisome proliferator-activated receptor γ; NF-κB, nuclear factor-kappaB; qRT-PCR, quantification real-time reverse transcription-polymerase chain reaction; HNPCs, human nucleus pulposus cells; IVD, intervertebral disc; NP, nucleus pulposus