Fig. 1

Decreased PPARγ expression in an IDD cell model induced by IL-1β. A qRT-PCR was used to detect the expression of PPARγ mRNA in HNPCs cultured with different concentrations of IL-1β for 48 h. B, C PPARγ protein level detected by Western blot after HNPCs were incubated in different concentrations of IL-1β for 48 h. D qRT-PCR was used to detect the expression of PPARγ mRNA after HNPCs were incubated at 10 mg/mL IL-1β for different durations. E, F PPARγ protein level detected by Western blot after HNPC were incubated at 10 mg/mL IL-1β for different durations. (*P < 0.05, **P < 0.01, ***P < 0.001 vs. previous nearby concentration group/time point group). PPARγ, peroxisome proliferator-activated receptor γ; mRNA, messenger RNA; IL-1β, interleukin-1β; IDD, intervertebral disc degeneration; qRT-PCR, quantification real-time reverse transcription-polymerase chain reaction; HNPCs, human nucleus pulposus cells