Fig. 3

Effects of ARHGAP44 on the invasion, migration, and apoptosis ability of MG63 and HOS cells, as detected using transwell invasion, wound healing, and flow cytometry assays. A Transwell invasion assay results for MG63 and HOS cells transfected with ARHGAP44-siRNA; B C The number of penetrating cells in the MG63 and HOS control groups was significantly higher than that in the ARHGAP44 knockdown group, ** for p < 0.01; D Experimental results of the control and ARHGAP44 knockdown groups at 0 h versus 24 h; E, F The healing area of the MG63 and HOS control group was larger than that of the ARHGAP44 knockdown group, ** for p < 0.01. G Flow cytometry assay results of the ARHGAP44 knockdown group and the control group; H, I The apoptosis ratio of MG63 and HOS control groups was significantly higher than that in the ARHGAP44 knockdown group, ** for p < 0.01, **** for p < 0.0001