Fig. 2

Knockdown cell model of ARHGAP44 was constructed and the effect of ARHGAP44 on the proliferative ability of MG63 and HOS cell lines was detected using CCK-8 and clonal formation assays. A, B Transfection efficiency was verified using qRT − PCR after siRNA transfection, ** for p < 0.01; C, D: Effect of ARHGAP44 expression inhibition on the cell activity of MG63 and HOS cell lines at 24, 48, 72, and 96 h, ** for p < 0.01; E Results of clone formation assay of MG63 and HOS cell lines after ARHGAP44-siRNA transfection; F, G Number of clonogenic cells in the MG63 and HOS control group was higher than that in the ARHGAP44 knockdown group, ** for p < 0.01