Fig. 1

Bioinformatic and immunohistochemical results for the detection of the differential expression of ARHGAP44 in osteosarcoma, correlation with tumor prognosis, and correlation with p53. A Differential gene analysis of GSE14359, as obtained using GEO database; B The expression level of ARHGAP44 in osteosarcoma tissues was higher than that in paracancerous tissues, p = 0.011; C Immunohistochemical results of ARHGAP44 in osteosarcoma tissue showing deeply stained brown–yellow nuclei, images were recorded using ×400 magnification; D Immunohistochemical positive expression of ARHGAP44 in paracancerous tissues was lower than that in tumor tissues, images were recorded using ×400 magnification; E: Kaplan–Meier survival curves of patients with high and low ARHGAP44-expressing osteosarcoma, p = 0.0291; F: ROC model for 1-, 3-, and 5-year survival in patients with high ARHGAP44 expression; G KM survival analysis established based on immunohistochemical results of clinical specimens, p = 0.0495; H TCGA database showing that ARHGAP44 expression in osteosarcoma was negatively correlated with p53, p = 2.04e−05, with a Spearman correlation coefficient of − 0.26; I–J: Western blotting results of ARHGAP44 expression in osteosarcoma and osteoblastic cell lines, * for p < 0.05, ** for p < 0.01