Fig. 6

SOX6 was a downstream target of miR-182. A miRDB was used to predict the binding relationship between miR-182 and 3′-UTR regions of SOX6. MM.1S and U266 cells were transfected with miR-182 mimic or with mimics NC. B miR-182 expression was detected in MM.1S and U266 cells by qRT-PCR. C The binding relationship between miR-182 and SOX6 3′-UTR was examined using the dual-luciferase assay. D, E qRT-PCR and western blot were performed to quantify the gene and protein expression levels of SOX6